Lysate RNase protection method for soft tissues
نویسندگان
چکیده
منابع مشابه
Lysate RNase protection method for soft tissues.
Estimations of RNA abundance in solid tissues by nuclease protection assays (1, 2) are difficult, because of problems attaining efficient RNA extraction and because of RNA instability during molecular hybridization or after denaturation of nuclease-treated, probe:target hybrids. A method to overcome these difficulties in the case of tissue cultured cells was published (3) which conducted molecu...
متن کاملSoft tissues
The deposition of amorphous calcium salts within the soft tissues is variously called mineralization or calcification. Two forms of calcium salts may be found in the soft tissues: calcium pyrophosphate dihydrate and calcium hydroxyapatite. If bony trabeculae are discernible within the mineralized focus the term ossification is used, sometimes prefixed with the terms ectopic or heterotopic. Ther...
متن کاملMethod of testing very soft biological tissues in compression.
Mechanical properties of very soft tissues, such as brain, liver, kidney and prostate have recently joined the mainstream research topics in biomechanics. This has happened in spite of the fact that these tissues do not bear mechanical loads. The interest in the biomechanics of very soft tissues has been motivated by the developments in computer-integrated and robot-aided surgery--in particular...
متن کاملA Novel Nonlinear Parameter Estimation Method of Soft Tissues
The elastic parameters of soft tissues are important for medical diagnosis and virtual surgery simulation. In this study, we propose a novel nonlinear parameter estimation method for soft tissues. Firstly, an in-house data acquisition platform was used to obtain external forces and their corresponding deformation values. To provide highly precise data for estimating nonlinear parameters, the me...
متن کاملDamage Models for Soft Tissues: A Survey
Damage to soft tissues in the human body has been investigated for applications in healthcare, sports, and biomedical engineering. This paper reviews and classifies damage models for soft tissues to summarize achievements, identify new directions, and facilitate finite element analysis. The main ideas of damage modeling methods are illustrated and interpreted. A few key issues related to damage...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.20.5492